Figure 1.

Adar 3/4 S overexpression causes lethality. (A) Survival of embryos, larvae and pupae of Adar 3/4 S OE at 25°C (n = 200) and at 27°C (n = 772) compared with w¹¹¹⁸ wild-type at 27°C (n = 400). Error bars represent Poisson 95% confidence intervals (B) Adar mRNA levels relative to Gapdh at 27°C versus 25°C in Adar 3/4 S OE whole embryos and L3 larvae. Three independent replicates were performed for qRT-PCR. The bars represent the mean value after normalizing to Gapdh expression levels. Error bars are SEM. Significance is indicated above the horizontal lines, *FDR P = 0.05, **FDR P = 1.841E-6 (two tailed Student’s t-test).