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. 2004 Mar 3;32(4):1555–1565. doi: 10.1093/nar/gkh304

Figure 3.

Figure 3

Confirmation of the 3′ end integration by sequencing the region of the 3′ junction between R2Bm and the host cell genome. The sequence around the boundary of the C65 28S rRNA gene is shown on the top of the diagram [(host) C65]. It was confirmed that this region is completely conserved in the N4 genome. The second sequence indicates the common region between sequences introduced into recombinant AcNPVs derived from the N4 strain [(rcAcNPV) N4] and the sequence of the 28S rRNA genes that was derived from C65 cells. Sequences below the diagram are those determined from C65 cell genomes infected by AcR2[5′|ORF|3′>30] and AcR2[ORF|3′>30]. Sequences derived from R2Bm were shown in the shaded box. One of 12 clones had four extra bases at the junction between R2Bm and the rRNA gene.