Figure 1.
SAXS and EM analysis reveals a V-shaped structure of Hop2-Mnd1. (a) P(r) of the Hop2-Mnd1 (red) computed from the experimental SAXS data (Supplementary Figure S1) compared with the P(r) obtained for (MBP-Hop2)-Mnd1, Hop2-(MBP-Mnd1) and (MBP-Hop2)-(MBP-Mnd1) assemblies (light, dark gray, cyan) and Hop21–84 construct (violet). The oscillations at 21, 42, ∼70 and ∼95 Å distances are indicated (black arrow). (b) Two views of the average SAXS envelope of the Hop2–Mnd1 assembly. The average envelope of the Hop21-84 construct (violet) is shown in proximity to the predicted N-terminal region in the Hop2–Mnd1 envelope. (c) Four (MBP-Hop2)-Mnd1 and Hop2-(MBP-Mnd1) multiphase envelopes calculated by MONSA are shown as a surface indicating the calculated phase for MBP (red) and Hop2–Mnd1 (yellow). Corespondiong multiphase fits to the experimental curves are shown in Supplementary Figure S1b. (d) Four (MBP-Hop2)-(MBP-Mnd1) multiphase envelopes calculated by MONSA are shown as a surface indicating the calcualted phase for MBP (red) and Hop2–Mnd1 (yellow). Corespondiong multiphase fits to the experimental curves are shown in Supplementary Figure S1b.