Figure 5.

Role of the N-terminal DNA binding domains of Hop2–Mnd1 in duplex DNA capture and synaptic complex assembly. (a) Schematic representation of the duplex capture assay. (b) WT and mutant variants of the Hop2–Mnd1 complex (0.5 µM) were tested for their ability to promote duplex capture with AMP-PNP as nucleotide cofactor. The mean values ± SD from three independent experiments were plotted. (c) Schematic representation the restriction enzyme protection assay to examine synaptic complex formation. (d) WT and mutant variants of the Hop2–Mnd1 complex (0.3 µM) were tested for their ability to promote synaptic complex formation with AMP-PNP as nucleotide cofactor. The mean values ± SD from three independent experiments were plotted.