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. 2013 Oct 21;42(2):918–925. doi: 10.1093/nar/gkt929

Figure 6.

Figure 6.

The C-terminal region of SMARCAL1 containing S889 is an auto-inhibitory domain. (A) Flag-SMARCAL1 proteins were purified from HEK293T cells and examined for ATPase activity. *P = 0.031; **P = 0.005; ***P = 0.0005. (B and C) U2OS cells expressing GFP-SMARCAL1-ΔC were treated with HU for 5 h, fixed and stained with antibodies to γH2AX and Flag. (C) U2OS cells expressing Flag-NLS-SMARCAL1-ΔC were treated with HU for 5 h, fixed and stained with antibodies to γH2AX and Flag. (D) The ATPase activities of Flag-SMARCAL1 proteins purified from HEK293T were examined in the presence of forked DNA. *P = 0.04; **P = 0.003. The insets in (A) and (D) are immunoblots of the purified proteins. (E) U2OS cells were transfected with FLAG-SMARCAL1 expression vectors and seeded onto coverslips. Two days after transfection the cells were fixed and stained with γH2AX and FLAG antibodies. Cells expressing Flag-SMARCAL1 proteins were scored for pan-nuclear γH2AX staining. Error bars denote standard deviation (n = 6). **P = 0.007; two-tailed unpaired t-test.