Figure 3.

Gel retardation assays of the binding of the Tat peptides to cTAR DNA. The cTAR ³²P-DNA was incubated in the absence or the presence of peptide and analyzed by electrophoresis on a 10% polyacrylamide gel as described in ‘Materials and Methods’. Lanes C1, heat-denatured cTAR DNA. Lanes C2, controls without peptide; lanes 1–4, peptide to nucleotide molar ratios were 1:4, 1:2, 1:1 and 2:1. The high molecular mass peptide:cTAR complexes (aggregates) are indicated by Ag.