Figure 5.

Evidence that Tat(44–61) does not promote cTAR/TAR RNA annealing in vitro. The 10 nM TMR-5′-cTAR-3′-Fl was reacted with 100 nM TAR RNA (black trace) or with 100 nM dTAR (green trace) using a peptide to oligonucleotide molar ratio of 3. For comparison, cTAR/TAR RNA annealing was also monitored in the presence of NC(11–55) at a molar ratio of 11 peptides per oligonucleotide (red trace). The solid lines correspond to the best fit of the kinetic curves to Equation (4).