Figure 3.

Northern analysis of mRNA levels in transfected cells. (A) Total RNA was prepared from cell cultures transfected with constructs uATG_b, uATG_d, a positive control (a construct with the same leader length but lacking a uAUG) and a negative control (untransfected control) as indicated on top of each lane. Similar intensities were detected for the positive control and uATG_b and uATG_d constructs when adjusted for RNA loading levels (28S and 18S RNA). (B) Northern hybridization of transcripts produced by constructs pEGFP-N1, pEGFP-N1-SL, uATG_b, uATG_bL and uATG_bL-SL transfected into HeLa cells. Similar GFP mRNA levels were seen for all transfected constructs.