Figure 4.

Conservation of the sequence in structural elements. (A) Reaction scheme showing the reaction catalyzed by MIRzymes. (B) Conserved four-way junction structure of a group of highly active MIRzymes (with reaction site highlighted in green). Frequency plots showing the sequence conservation of two single-stranded bulge regions (s6 and s8) in the conserved four-way junction structure, analyzed using RNABOB, as described in Supplementary Text S6. The bar-graph depicts normalized reactivity for all RNA19 mutants, relative to RNA19 (average of triplicate, S.D. <5% of average wild-type activity, except s6, position 5 A to U – 10.8%).