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. 2013 Oct 24;42(2):860–869. doi: 10.1093/nar/gkt957

Figure 4.

Figure 4.

The 1H6 foci decrease in the presence of G4 DNA and increase in the presence of TMP and TMS. (a) Synthetic tetramolecular G4 DNA (Oxy-2 tetraplex) decreases 1H6 nuclear foci as detected by flow cytometry. Human HeLa cells were prepared and stained with anti-G-quadruplex antibody 1H6 with and without the presence of synthetic tetramolecular G4 DNA. Mean fluorescence was measured by flow cytometry and plotted above. Incubating the 1H6 antibody in the presence of tetramolecular DNA decreases the total mean fluorescence (n > 25 000). (b) Flow cytometry quantitative analysis of HeLa cells show increased mean fluorescence on treatment with TMPyP4. HeLa cells treated with increasing concentrations of TMPyP4 and subsequently stained with the 1H6 antibody. Increased TMPyP4 treatment resulted in increased mean fluorescence detected for 1H6 but not controls. TMPyP4 concentrations (TMP) in µM are listed below (n > 25 000). (c) Flow cytometry analysis of HeLa cells treated with TMPyP4 or no drug control. Log scale differences observed on treatment in cells stained with 1H6 as compared with controls (n > 20 000).