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F349 and Y409 of Rad52 are essential for the interaction with Rad51. Size exclusion chromatography analyses of Rad52233–504 F349A-Rad51 (A) and Rad52233–504 Y409A-Rad51 (B) mixtures. The elution profiles of the proteins from a Superdex 200 gel filtration column were monitored by the absorbance at 280 nm. The boxed portion (9.5–14 ml) containing the peak was fractionated in 0.5 ml portions, and each fraction was analyzed by SDS-PAGE (lanes 1–9), as shown below.
