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. 2013 Oct 21;42(2):1291–1302. doi: 10.1093/nar/gkt987

Figure 4.

Figure 4.

Effect of SRSF4 on normal spliced GAA mRNA in fibroblasts. (A) P1 fibroblasts were transfected with 2–4 ug of SRSF4, and the relative amount of normal spliced GAA mRNA was assessed by real time PCR. Results are expressed as percentage of the normal variant detected in fibroblasts transfected with the empty vector (mock). Data are means ± SD of three independent experiments. *P < 0.05. (B) Western blot analysis of endogenous SRSF4 protein expression in primary cultured fibroblasts from a healthy donor after knocking down the expression of SRSF4 by siRNA. (C) Relative abundance of the normal GAA variant assessed by real time PCR in cultured fibroblasts from a healthy donor after knocking down the expression of SRSF4 by siRNA. Results are expressed as percentage of the normal variant detected in fibroblasts transfected with the scramble siRNA. Data are means ± SD of three independent experiments. *P < 0.05.