Figure 6.

Effect of HDAC inhibitors on GAA splicing. Cells transfected with the MUT minigene were treated for 24 h with sodium butyrate, phenylbutyric acid and SAHA. The relative expression of the N isoform was analyzed by real time PCR, using minigene-specific primers designed to amplify only this variant. The results are expressed as fold of N isoform detected in untreated cells. The abundance of the N isoform produced by cells transfected with the WT minigene is also shown for comparison. Data represent the means ± SD of three independent experiments. *P < 0.05.