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Transient subcellular localization of a constitutively expressed EGFP-R1a fusion upon UV-C irradiation. The EGFP:R1a fusion protein was stably expressed in transgenic BY-2 cells under the control of a 35S promoter. Fluorescence was observed in non-irradiated cells (C) or in cells at 3, 4 and 5 h following UV-C irradiation (450 J/m2). LMB (L) was added overnight (0.1 µM) before cell irradiation. These photographs are representatives of four individual experiments. Bar = 10 µm.
