Table 1.
Transcripts exhibiting a differential response for Qfhs.ifa‐5A a (adjusted P ≤ 0.05, ≥2 fold change, FC) after inoculation with Fusarium graminearum spores
| Affymetrix ID | Annotationb | Classification | FC, 8 haic | FC, 24 hai | FC, 72 hai | Significant in other contrastsd | Mapped toe |
|---|---|---|---|---|---|---|---|
| Ta.9975.2.S1_at | Unknown | Unknown | 1.51 | 0.95 | 6.28 | ||
| Ta.14573.1.S1_at | Hsp20/α‐crystallin family protein (Oryza sativa) | Defence | 0.23 | 0.80 | 1.68 | ||
| Ta.13859.1.A1_at | OsCML16; calmodulin‐related calcium sensor protein (Oryza sativa) | Regulatory | 2.94 | 0.64 | 1.30 | −Fhb1 (24 hai) | |
| Ta.16827.1.S1_s_at | Unknown | Unknown | 0.75 | 0.35 | 1.25 | ||
| Ta.10311.1.S1_at | Stress‐responsive A/B barrel domain‐containing protein (Oryza sativa) | Unknown | 2.65 | 1.01 | 1.16 | ||
| Ta.13426.1.S1_s_at | Os04g0119500 protein (Oryza sativa) (UniRef90_Q7XTJ2) | Unknown | 3.14 | 0.89 | 1.09 | ||
| TaAffx.64985.1.S1_at | Putative uncharacterized protein (Zea mays) (UniRef90_B4FQP2) | Regulatory | 0.17 | 2.02 | 1.00 | ||
| Ta.9389.1.S1_at | Seed maturation protein PM41 (Oryza sativa) | Development | 2.20 | 0.31 | 0.98 | ||
| Ta.20205.1.A1_s_at | VER2 (Triticum aestivum) (UniRef90_O80370) | Development | 1.14 | 0.48 | 0.95 | ||
| Ta.24801.1.S1_at | Hsp20/α‐crystallin family protein (Oryza sativa) | Defence | 0.25 | 0.95 | 0.90 | ||
| Ta.5004.1.S1_at | Thiol protease SEN102 precursor (Oryza sativa) | Metabolism | 0.26 | 1.70 | 0.89 | −Fhb1 (8 hai) | |
| Ta.5004.3.S1_a_at | Thiol protease SEN102 precursor (Oryza sativa) | Metabolism | 0.47 | 1.19 | 0.88 | −Fhb1 (8 hai) | |
| TaAffx.112045.1.S1_x_at | Glutathione‐S‐transferase (Oryza sativa) | Defence | 3.20 | 1.23 | 0.03 | +Fhb1 (8 hai) | |
| Ta.5610.1.S1_at | Nonsymbiotic haemoglobin 2 (Oryza sativa) | Miscellaneous | 7.01 | 0.40 | 0.02 | 4BL, 4DL |
The expression ratio was calculated from the F. graminearum/mock response observed in NIL3 (resistant Qfhs.ifa‐5A allele) and the response provided by NIL4 (susceptible allele). Genes more abundant in the absence of Qfhs.ifa‐5A at 72 hai are excluded here and provided in Table S3 (see Supporting Information).
Annotations were assigned if the E value from the BlastX results was ≤10−10; otherwise the transcripts were denominated as ‘unknown’.
Differences in response are the means of three biological replicates. Statistically significant values are given in bold type.
−/+ indicates significant positive/negative differences in response for the indicated contrast.
Map locations were identified by searching the mapped expressed sequence tag (EST) database of Graingenes for the probe set sequences using BlastN and an E cut‐off of ≤10−10.