Table 3.
Transcripts exhibiting a differential response for Fhb1 a (adjusted P ≤ 0.05, ≥2 fold change, FC) after inoculation with Fusarium graminearum spores
| Affymetrix ID | Annotationb | Classification | FC, 8 haic | FC, 24 hai | FC, 72 hai | Significant in other contrastsd | Mapped toe |
|---|---|---|---|---|---|---|---|
| Ta.14071.1.S1_x_at | Integral membrane protein DUF6‐containing protein, expressed (Oryza sativa) | Metabolism | 1.37 | 1.24 | 3.17 | ||
| Ta.13859.1.A1_at | OsCML16; calmodulin‐related calcium sensor protein (Oryza sativa) | Regulatory | 1.12 | 0.20 | 1.28 | −Qfhs.ifa‐5A (8 hai) | |
| TaAffx.616.2.S1_s_at | Hypothetical protein (Jia et al., 2009) | Unknown | 1.49 | 0.27 | 1.16 | ||
| TaAffx.112045.1.S1_x_at | Glutathione‐S‐transferase (Oryza sativa) | Defence | 3.26 | 1.58 | 1.11 | + Qfhs.ifa‐5A (8 hai) −Qfhs.ifa‐5A (72 hai) | |
| Ta.5004.1.S1_at | Thiol protease SEN102 precursor (Oryza sativa) | Metabolism | 0.29 | 1.52 | 0.83 | −Qfhs.ifa‐5A (8 hai) | |
| Ta.5004.3.S1_a_at | Thiol protease SEN102 precursor (Oryza sativa) | Metabolism | 0.41 | 1.24 | 0.79 | −Qfhs.ifa‐5A (8 hai) | |
| TaAffx.106424.1.S1_at | Acid phosphatase‐like (Jia et al., 2009) | Metabolism | 0.94 | 1.13 | 0.49 | −Qfhs.ifa‐5A (8 hai) | |
| Ta.8866.1.S1_at | Protease inhibitor‐like protein (Jia et al., 2009) | Defence | 1.03 | 0.95 | 0.44 | −Qfhs.ifa‐5A (8 hai) | |
| Ta.25754.1.A1_at | Glutathione‐S‐transferase (Oryza sativa) | Defence | 0.99 | 0.96 | 0.39 | −Qfhs.ifa‐5A (8 hai) | |
| Ta.1811.1.S1_at | cis‐Zeatin O‐glucosyltransferase (Oryza sativa) | Putative trichothecene detoxification and transport | 0.94 | 0.89 | 0.39 | −Qfhs.ifa‐5A (8 hai) | |
| Ta.10583.1.A1_at | tRNA synthetases class II domain‐containing protein (Oryza sativa) | Metabolism | 0.90 | 1.02 | 0.38 | −Qfhs.ifa‐5A (8 hai) | 1AS, 1BS, 1 DS |
| TaAffx.113333.2.S1_at | Putative uncharacterized protein Sb04g011090 (Sorghum bicolor) (UniRef90_C5Y052) | Metabolism | 0.93 | 1.15 | 0.36 | −Qfhs.ifa‐5A (8 hai) | 1AL, 1BL, 1DL, 5DL |
| Ta.30866.1.S1_at | Expressed protein‐RZ53 family‐2 (Oryza brachyantha) (UniRef90_B9V0I1) | Unknown | 0.94 | 1.04 | 0.36 | −Qfhs.ifa‐5A (8 hai) | |
| Ta.3545.1.S1_at | Unknown | 0.98 | 1.04 | 0.30 | −Qfhs.ifa‐5A (72 hai) | 3AL, 3BL, 3DL | |
| Ta.24732.1.S1_at | Unknown | 1.20 | 1.21 | 0.16 | −Qfhs.ifa‐5A (8 hai) | 1BL, 3BL, 6DL, 7BL | |
| Ta.23324.1.S1_at | Unknown | 0.94 | 1.13 | 0.02 |
The expression ratio was calculated from the F. graminearum/mock response observed in NIL2 (resistant Fhb1 allele) and the response provided by NIL4 (susceptible alleles at Fhb1).
Annotations were assigned if E from the BlastX results was ≤10−10; otherwise, the transcripts were denominated as ‘unknown’.
Differences in response are the means of three biological replicates. Statistically significant values are given in bold type.
‐/+ indicates significant positive/negative differences in response for the indicated contrast.
Map locations were identified by searching the mapped expressed sequence tag (EST) database of Graingenes for the probe set sequences using BlastN and E ≤ 10−10.