Representative PCR amplification of three distinct CD22 transcript regions using cDNA generated from the designated cell lines (1- Daudi; 2 - Jurkat; 3 - HCC827; 4 - H1355; 5 - H1975; 6 - A549; 7 - Calu-1; 8 - H1650; 9 - H727; 10 - dH2O). Daudi and Jurkat cells served as positive and negative controls, respectively. After 30 cycles of amplification, faint bands corresponding to CD22 transcripts were observed for Calu-1, H727, HCC827, H1650 and H1975 relative to the control (Daudi cells). Similar results were obtained in three or more experiments.