Fig. 1.

Characterisation of DC. a MHC I H-2^k and H-2^b expression on C57BL/6 or F1-cross derived mature DC was determined by FACS analysis. 54% of C56BL/6 DC (MFI 114) and 62% of F1 DC (MFI 74) had high levels of H-2^b expression. b Markers of DC phenotypic maturation; MHC II, CD80 and CD86 were also determined by FACS analysis. Figures shown are percentages of all positively stained cells (F1 DC black; C57BL/6 DC grey). c Functionally mature DC from C57BL/6 (hatched bar) and F1 (black bar) mice were compared in their ability to stimulate a proliferative response in third party splenocytes from BALB/c mice (H-2^d). Proliferation was measured by 18 h ³H-thymidine incorporation. Results shown from one representative assay from three separate experiments and are mean values from sextuplet experimental wells