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WAVE™ System DHPLC traces of self-cleavage reactions showing analysis of different clones or the selected pool. The reactions were performed for 1 h at 37°C in 50 mM Tris–HCl, pH 7.5; 10 mM EDTA; 0.2 M NaCl. ‘DNA pool’ means enriched pool of molecules after the last selection round; ‘Family 1’, one clone from family 1 sequences; ‘Family 2’, two different clones (clone 32 and clone 36) from family 2 sequences. Clone 32 (family 2) was the most active.
