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. 2014 Jan 22;7:58. doi: 10.1186/1756-0500-7-58

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Copyright © 2014 Garg et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Real time PCR analysis of EPSPS gene against glyphosate stress. qPCR analysis of EPSPS transcript showing their regulation in response to glyphosate treatment (A). Real time PCR analysis of EPSPS during post treatment of 2.16 mg/l glyphosate at different time points. (B). Relative mRNA expression of EPSPS gene in different parts of plant. The relative mRNA expression for each transcript was calculated in comparison to control plants. Bars indicate the standard error (± SE) calculated from three independent experiments.