Figure 1. Higher diastolic, systolic, and resting [Ca²⁺]_i and larger sarcolemmal leak in TS cells.

A–B, representative [Ca²⁺]_i (nM) traces of WT (black) and TS (red) cells in diastole, systole, and at rest measured using the indicators ACaR (A) and Fluo-4 (B). C, bar plots of the mean ± S.E.M. of the diastolic, systolic, and resting [Ca²⁺]_i (nM) levels of WT and TS cells measured using each indicator. D, representative time courses of WT (black) and TS (red) cell sarcolemmal [Ca²⁺]_i (nM) leak (upon application of thapsigargin and removal of Na⁺ and Ca²⁺ from bath) and recovery (upon restoration of 2 mM Ca²⁺ to bath). Grey and pink traces indicate same experiment upon application of nifedipine.