Thymus, spleen, and liver NKT cells numbers were calculated by flow cytometry
after staining with anti-TCRβ and CD1dtetramer/PBS57. Representative
FACS contour plots depicting the percentages of NKT cells in the different
organs are shown at top. The NKT cell population is circled, and the percentages
are indicated. Percentages for thymus and spleen are of total cells, while
percentages for liver are of CD45+ IHLs. Cumulative
percentages and numbers of NKT cells in the different organs are shown at
bottom. Data represent the means ± s.d., n = 4–5 mice
per strain, *p ≤ 0.01, **p ≤ 0.01,
***p ≤ 0.001.