(A) Comparison of CD1d expression among the B6.PWDchr3
consomic strain and the B6 and PWD parental strains. Representative thymocyte
histograms are shown at left. Shaded histograms represent unstained controls,
while open histograms represent anti-CD1d staining. The percent positive cells
are indicated. Relative thymocyte and splenocyte CD1d fluorescence is shown at
right. Relative CD1d fluorescence was calculated as specified in Materials and
Methods. Data represent the mean relative fluorescence ± s.d., n
= 3 – 4 mice per strain, *p ≤ 0.05,
**p ≤ 0.01, ***p ≤
0.001. (B) Representative FACS contour plots depicting the
percentages of NKT cells in different organs are shown at left. The NKT cell
population is circled, and the percentages are indicated. Cumulative percentages
and numbers of NKT cells in the organs of different strains are shown at right.
Data represent the means ± s.e.m., n = 5–7 mice per
strain, *p ≤ 0.01, **p ≤ 0.01,
***p ≤ 0.001.