(A) Comparison of serum cytokine levels among strains 90 min after
i.p. αGalCer administration. (B) Comparison of ex
vivo splenocyte cytokine production 90 min. after i.p.
αGalCer administration. (C) Representative FACS data
depicting intracellular cytokine staining by spleen NKT cells after i.p.
αGalCer administration. Histograms depict intracellular cytokine
staining of gated NKT cells. Shaded histograms represent isotype-matched control
mAb staining, while open histograms represent the anti-cytokine staining. The
percentage of NKT cells positive for cytokine production is indicated.
Intracellular cytokine production in PWD NKT cells could not be determined
because of the low number of cells. Cumulative MFIs of NKT cell intracellular
cytokine staining is shown at right. Data represent the mean MFI ± s.d.,
n = 3–4 mice per strain, *p ≤ 0.01,
**p ≤ 0.01, ***p ≤
0.001.