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. 2004 Feb 24;32(4):e42. doi: 10.1093/nar/gnh039

Table 1. The DNAs from the apoE gene and PNA probes used for the present genotyping^a,b.

Sense strands in apoE gene
(1) 112 codon ^(Sense)	203mer
apoE^(S112T) (112Cys)	5′···(3925)ATGGAGGACGTGTGCGGCCGCCTGGTG(3951)···3′
apoE^(S112C) (112Arg)	5′···(3925)ATGGAGGACGTGCGCGGCCGCCTGGTG(3951)···3′
PNA^(S112A)	H₂NCO-(Gly)-TGCACACGCC-NH₂ 10mer
PNA^(S112G)	H₂NCO-(Gly)-TGCACGCGCC-NH₂ 10mer
(2) 158 codon ^(Sense)	106mer
apoE^(S158T) (158Cys)	5′···(4063)GACCTGCAGAAGTGCCTGGCAGTGTAC(4089)···3′
apoE^(S158C) (158Arg)	5′···(4063)GACCTGCAGAAGCGCCTGGCAGTGTAC(4089)···3′
PNA^(S158G)	H₂NCO-(Gly)-TCTTCGCGGA-NH₂ 10mer
Antisense strands in apoE gene
(1) 158 codon ^(Antisense)	203mer
apoE^(A158A) (158Cys)	5′···(4089)GTACACTGCCAGGCACTTCTGCAGGTC(4063)···3′
apoE^(A158G) (158Arg)	5′···(4089)GTACACTGCCAGGCGCTTCTGCAGGTC(4063)···3′
PNA^(A158T)	H₂NCO-(Lys)-GTCCGTGAAGAC-NH₂ 12mer
PNA^(A158C)	H₂NCO-(Lys)-GTCCGCGAAGAC-NH₂ 12mer

^aThe underlined part of DNA substrate is complementary with the PNA probe, and the polymorphic nucleotide is in bold.

^bThe sequence data are from DDBJ/EMBL/GenBank accession no. AF261279.