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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Virology. 2013 Sep 7;446(0):293–302. doi: 10.1016/j.virol.2013.07.011

Fig. 2.

Fig. 2

Purification and SDS-PAGE profiles of the T4 wild type and portal gp20 A316C mutant proheads. (A) Wild type and gp20 A316C proheads produced predominantly mature empty large proheads (elps) with a smaller amount of empty small proheads (esps) as seen by the linear salt gradient (straight brown line) purification profiles on FPLC-DEAE columns. The third peak in the chromatogram is nucleic acid (NA). Cyan, green, and purple are the 214, 280, and 260 nm traces respectively. (B) elps and esps are distinguished by release of the major capsid protein (mcp) gp23 into the gel by not heating (−) or heating (+) prohead samples prior to 10% SDS-PAGE electrophoresis followed by Coomassie staining.