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. Author manuscript; available in PMC: 2014 Nov 20.
Published in final edited form as: Bioconjug Chem. 2013 Nov 7;24(11):1784–1789. doi: 10.1021/bc400322h

Figure 3.

Figure 3

Synthesis and analysis of bioorthogonal pHLIP-Tz. (A) Synthesis of Tz-maleimide; Reagents and conditions: (i) maleic anhydride, BOP, Et3N, room temperature, 4.5 h, 34%; (B) HPLC and ESI-MS traces of Tz-maleimide; (C) Synthesis of pHLIP-Tz from Tz-maleimide Reagents and conditions: (ii) pHLIP, PBS, room temperature, 3 h, 77%; (D) HPLC and ESI-MS traces of pHLIP-Tz.