Fig. 4.
Variability in kinetic properties of HO-2. (A) Effect of graded hypoxia on CO generation in liver microsomal fractions in SD, BN, and SH rats. (B) Analysis of half-maximal initial rate of CO generation using Eadie-Hofstee plot. (C and D) Apparent Km for hemin (C) and Vmax (D) of HO-2-catalyzed CO generation in liver microsomes from SD, BN, and SH rats. Data represent mean ± SEM from 5 individual experiments. **P < 0.01; ***P < 0.001; n.s. P > 0.05 (i.e., not significant). See also Fig. S5.