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. 2014 Jan 6;111(3):960–965. doi: 10.1073/pnas.1317400111

Fig. 3.

Fig. 3.

Functional characterization of transport reactions catalyzed by recombinant UCP2. (A) Uptake of radioactive substrates into liposomes reconstituted with recombinant UCP2 and UCP1 and containing the same unlabeled substrate (n = 3). (B) Efflux of labeled substrates from preloaded proteoliposomes (n = 3). (C) 33Pi/Pi exchange activity in liposomes reconstituted with yeast mitochondrial extracts (n = 3). (D) Swelling of yeast mitochondria in isosmotic ammonium solutions of various anions. (E) Effect of FCCP on 33Pi/malate and l-[14C]malate/malate exchange reactions in proteoliposomes reconstituted with UCP2. FCCP (5 μM) was added together with the labeled substrate (n = 5). All error bars denote ±SEM (*P < 0.0006, t test).