Fig. 7.
Contactin regulates nodal sodium channel development. Sodium channel clustering in the optic nerve was disturbed in Cntn1-KO at P18. (A) WT and KO optic nerve sections were stained for Nav1.6 (green) and NaCh pan (red). Fewer total number of nodes (red images compared), fewer mature nodes (green images compared), and more elongated sodium channel clusters (red images compared) were observed on KO compared with WT. (Scale bar, 5 µm.) (B) Quantification shows reduced number of NaCh clusters per area unit in Cntn1-KO compared with WT (*P < 0.005 WT n = 5, KO n = 4). (C) KO samples contain reduced number of sodium channel clusters expressing Nav1.6 (*P < 0.005 WT n = 5, KO n = 4).