FIG 7 .

ROCK is a downstream effector of Erk1/2 in HSV-1-induced early cofilin phosphorylation. (A) ROCK activation during HSV-1 entry. The cells were infected with HSV-1 (MOI 20), and the cell lysates were analyzed with an anti-ROCK antibody. The ROCK inhibitor, Y-27632 (100 µM), was used to confirm its effect on HSV-1 entry. (B) PAK1/2 is not involved in HSV-1 entry, and the PAK1/2-specific inhibitor IPA-3 (20 µM) did not affect virus entry or binding. (C) Kinase inhibitors block the activation of ROCK with no effect on PAK1/2. Inhibitor concentrations were similar to those used before. (D) Arp2/3 complex inhibitor CK548 does not affect HSV-1 entry, binding, or postentry. (E) Simple schematic of the signaling pathway involved in HSV-1-induced early cofilin phosphorylation.