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Caffeine- and aspartame-dependent RiboReporter™ sensor assays. (A) Measurement of fluorescence at 535 nm versus time at various concentrations of caffeine (S2.caf.D11) or aspartame (S3.asp.E4). The activity of clone S3.asp.E4 was determined in assay buffer at pH 7.5 in the presence of 40 mM MgCl2, and clone S2.caf.D11 was tested in assay buffer at pH 7.0, 10 mM MgCl2. (B) Sensor cleavage rates versus effector concentration calculated as described in Materials and Methods.
