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. 2004 Mar 12;32(5):1721–1730. doi: 10.1093/nar/gkh338

Figure 1.

Figure 1

Cap-independent translation of TNV sgRNA2 in wheat germ extract requires the leader and a 140 nt sequence in the trailer. (A) Schematic representation of TNV sgRNA2 and the deletion mutants. The box (nucleotides 153–983) indicates the coat protein open-reading frame. The gray and the hatched parts of the box (nucleotides153–737 and 738–983, respectively) indicate those parts of the coat protein coding region that are within or downstream of, respectively, the open-reading frame in the frameshift mutant sgRNA2fs. (B) TNV sgRNA2 is translated cap independently in wheat germ extract. Capped and uncapped versions of TNV sgRNA2 were translated in wheat germ extract and the TE was calculated. (C) Both the leader and the trailer of TNV sgRNA2 contain sequence information required for cap-independent translation. TE of TNV sgRNA2 derivatives with both leader and trailer (full-length sgRNA2), lacking the trailer, lacking the leader, or lacking both leader and trailer was determined in wheat germ extract. (D) Nucleotides 1152–1224 of TNV sgRNA2 are not required for cap-independent translation. 3′ Deletion derivatives of TNV sgRNA2 were translated in wheat germ extract and the TE was determined. (E) Nucleotides 738–1011 are not required for cap-independent translation. TE of TNV sgRNA2 frameshift mutants without (full-length sgRNA2fs) or with different 5′ deletions of the trailer in wheat germ extract. The indicated numbers refer to the deleted TNV sgRNA2 nucleotide. All nucleotides are numbered according to their position in the full-length TNV sgRNA2 (4).