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. 2004 Mar 12;32(5):1721–1730. doi: 10.1093/nar/gkh338

Figure 4.

Figure 4

TNV sgRNA2 leader and BYDV trailer functionally interact. TE of uncapped RNAs containing vector-derived UTR sequences (vector), TNV sgRNA2 UTR sequences (TNV) or BYDV UTR sequences (BYDV) in wheat germ extract. Stimulation by viral elements is the increase in TE as compared with the RNA lacking viral sequences (vector–vector). The sum of the individual elements is the sum of (the stimulation of the 5′ viral element with the vector sequences as 3′ element) and (the stimulation of the 3′ viral element with the vector sequences as 5′ element). Synergism between viral 5′ and 3′ UTR sequences is observed when the stimulation of the two elements together is higher than the sum of the stimulation by the individual elements (30).