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. 2004 Mar 16;32(5):1767–1773. doi: 10.1093/nar/gkh339

Figure 1.

Figure 1

Complementation efficiency of human TOP2α expressed from YCp-PADH1. JN394t2-4 cells were transformed with wild-type or 12 Pro mutants in the centromeric YCp-PADH1. Solid bars show the relative colony numbers at 35/27°C (permissve/non-permissive temperature), with mutated residues indicated. Complementation efficiency of M766P varied in experiments (10–60%). For mutants with high complementation efficiency, the data represent the averages of 3–7 experiments and are associated with S.D. ranges. Amino acids in the putative α4 helix are boxed.