Table 1.

Basic electrophysiological properties of Purkinje cells

	Membrane capacitance (pF)a,d	Resting membrane potential (mV)a	Input resistance (MΩ)a,d	Percentage of cells showing spontaneous action potentialsb,e	Frequency of spontaneous action potentials (Hz)c,e
GFP alone	39.2 ± 24.3	−58.3 ± 13.6	379 ± 278	80%	0.43 ± 1.00
	(n= 35)	(n= 35)	(n= 35)	(12 of 15 cells)	(n= 15)
WT mKv3.3	40.1 ± 16.1	−59.9 ± 8.24	367 ± 181	75%	0.25 ± 0.59
	(n= 17)	(n= 17)	(n= 17)	(6 of 8 cells)	(n= 8)
R424H mutant	22.3 ± 6.9*†	−59.5 ± 7.4	372 ± 169	64%	0.12 ± 0.75
	(n= 28)	(n= 28)	(n= 28)	(9 of 14 cells)	(n= 14)

Here and in Table 2, statistical analysis was conducted between cells expressing the R424H mutant and those expressing green fluorescent protein (GFP) alone or between cells expressing R424H mutant and those expressing wild-type (WT) mKv3.3. Data are given as the means ± SD, and n is the number of experiments. ^*P < 0.001 between GFP alone and R424H mutant. †P < 0.001 between WT mKv3.3 and R424H mutant. The statistical analysis indicated by superscript letters a, b and c was conducted using Student’s unpaired t test, the χ² test and Mann–Whitney U test, respectively. ^dMembrane capacitance and input resistance were measured in voltage-clamp conditions. ^eSpontaneous action potentials were recorded at resting membrane potential for 300 s.