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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Immunol. 2013 Nov 22;192(1):200–205. doi: 10.4049/jimmunol.1302289

Fig. 4. Functional memory is generated even from low affinity T cells primed in an environment with limited inflammation.

Fig. 4

(A) More than 30 days after the primary response (as described in Fig. 1), mice of all 4 experimental groups were immunized with recombinant vaccinia virus expressing Ova (VAC-OVA) and T cell responses in (A) the spleen and (B) lung were determined 5 days later. (C) Memory cell function was assessed in an in vitro restimulation assay by measuring IFNγ and granzyme B production. Data shown are representative for 2 independently performed experiments with a minimum of 3 mice per group. Similar data was obtained from 2 experiments using recombinant vesicular stomatitis virus (VSV-OVA). Statistical significance was determined by student t-test. p-values >0.05 were considered not significant (ns), 0.01 to 0.05 significant (*), 0.001 to 0.01 very significant (**) and <0.001 extremely significant (***).