Figure 2. Treatment with Y27632 causes profound disruption of the actin cytoskeleton.
MDA-MB-231 and DU-145 were incubated for 48 hours alone (control) or with Y27632 (150 µM for 24 or 48 hours with MDA-MB-231 cells or 100 µM for 24 or 48 hours with DU-145 cells). The cells were stained with conjugated phallotoxin binding to F-actin and photographed. The representative photos are displayed with DAPI staining representing nuclei.