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. 2004 Mar 19;32(5):1808–1817. doi: 10.1093/nar/gkh348

Figure 5.

Figure 5

Effect of upstream ATGs in BACE-1 transcript leader on translation efficiency. (A) Scheme of the long BACE-1 transcript leader in the bi-monocistronic plasmid. The four upstream ATGs are marked with arrowheads. The black box represents the upstream ORF. The upstream ATGs were mutated to TTG, generating pBRm2L-B1x-mut1, pBRm2L-B1x-mut2, pBRm2L-B1x-mut3. (B) Transient transfection of bi-monocistronic plasmids in SK-N-BE cells. Results are normalized to the value of the long BACE-1 leader (pBRm2L-B1x).