The effect of knockdown of GCNT2 on cell proliferation, colony formation, cell proliferation, and invasion. A, knockdown of GCNT2 in 4T1 cells with shRNA. Two clones (shRNA1 and shRNA5) show significant reduction of GCNT2 expression. B, two clones with significant GCNT2 expression knockdown show a marked decrease in cell proliferation rates compared with nontarget control by MTT assay. C, two clones with significant GCNT2 expression knockdown show a marked decrease in colony formation compared with nontarget control. D, two clones with significant GCNT2 expression knockdown show a marked decrease in migration (left) and invasion (right) compared with the nontarget control.