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. Author manuscript; available in PMC: 2014 Jan 27.
Published in final edited form as: Cancer Res. 2011 Jul 12;71(14):4846–4856. doi: 10.1158/0008-5472.CAN-11-0414

Figure 6.

Figure 6

The involvement of GCNT2 in TGF-β signaling. A, induction of GCNT2 by TGF-β1. NMuMG cells were treated with 5 ng/mL TGF-β1 for 2 days. GCNT2 expression is confirmed by quantitative real-time RT-PCR. B, knockdown of GCNT2 in NMuMG cells with shRNA was confirmed by RT-PCR (top) and real-time RTPCR (bottom). C, representative pictures show the morphologic change after treatment of TGF-β1 in NMuMG cells with nontarget vector and shRNA1. D, Western blotting results show the effect of GCNT2 on E-cadherin expression after TGF-β1 treatment in NMuMG cells with nontarget control and shRNA1. The lower panel shows the quantification of the signal intensity.