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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Eur J Neurosci. 2013 Sep 16;38(11):3602–3610. doi: 10.1111/ejn.12359

Fig. 5.

Fig. 5

Expression of VGluT2 mRNA in the RRF (–6.48 mm from bregma) (radioactive in situ hybridization). Coronal sections were incubated with anti-TH antibodies and hybridized with VGluT2 antisense radioactive riboprobes. (A) TH neurons are seen as brown cells and VGluT2 neurons as green grain aggregates (arrows) under bright-field/epiluminescence microscopy. (A′) These VGluT2 neurons are seen as silver grain aggregates under epiluminescence microscopy (arrows). Note numerous VGluT2 neurons intermingled with TH immunoreactivity (brown cells) throughout the RRF. (A″) TH cells after silver grains were removed. The scale bar shown in B″ is 750 μm for A and A′, and 100 μm for B and B″.