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. 2014 Jan 27;9(1):e85727. doi: 10.1371/journal.pone.0085727

Figure 4. Thin layer chromatography (TLC) analyses of biotransformation of Rb1, Rd, Gyp XVII, Rg3(S) in (A) and Rb2, Rb3, Rc in (B) by recombinant BglPm.

Figure 4

The reaction time was 30: CHCl3-CH3OH-H2O (65∶35∶10, lower phase). (S, S1, S2, S3), ginsenoside standards (PPD type ginsenoside mixtures); Standards: 1, 3, 5, 7, 9, 11, 13; Reaction mixture: 2, 4, 6, 8, 10, 12, 14; 1, Rb1; 2, reaction mixture of Rb1; 3, Rd; 4, reaction mixture of Rd; 5, Gyp XVII; 6, reaction mixture of Gyp XVII; 7, Rg3(S); 8, reaction mixture of Rg3(S); 9, Rb2; 10, reaction mixture of Rb2; 11, Rb3; 12, reaction mixture of Rb3; 13, Rc; 14, reaction mixture of Rc. Abbreviations: C-K, compound K.