Table 1. Effects of metal ions and chemical agents on the activity of purified recombinant BglPm.
Relative activity | ||
1 mM | 10 mM | |
NaCl | 98.8±2.5 | 100.3±1.4 |
KCl | 99.0±1.3 | 100.2±2.2 |
MgCl2 | 88.3±0.9 | 57.0±4.7 |
MnCl2 | 87.1±2.2 | 126.0±6.6 |
CaCl2 | 94.6±3.6 | 85.9±1.2 |
ZnCl2 | 98.3±1.3 | 78.3±1.5 |
CoCl2 | 82.9±3.3 | 85.0±6.1 |
CuCl2 | 70.1±6.4 | 105.3±3.6 |
HgCl2 | 22.7±1.5 | NDa |
SDS | ND | ND |
EDTA | 102.6±5.4 | 86.3±4.8 |
β-Mercaptoethanol | 101.5±6.3 | 86.5±7.1 |
DTT | 96.8±3.5 | 80.14±4.2 |
Control | 100±2.4 | 100±1.9 |
Not determined.