Table 2. Concentration-dependent virucidal activity of 5 biocides against animal parvoviruses.
minute virus of mice | porcine parvovirus | bovine parvovirus | canine parvovirus | ||||||||||||
Lab 3 | Lab 4 | Lab 1 | Lab 3 | Lab 5 | Lab 4 | Lab 2 | |||||||||
log10 TCID50/ml | log10 RF ±95%CI | log10 TCID50/ml | log10 RF ±95%CI | log10 TCID50/ml | log10 RF ±95%CI | log10 TCID50/ml | log10 RF±95%CI | log10 TCID50/ml | log10 RF±95%CI | log10 TCID50/ml | log10 RF±95%CI | log10 TCID50/ml | log10 RF±95%CI | ||
Control | inocculum | 5.80 | 8.03 | n.t. | 5.42 | n.t. | |||||||||
t0 min | 5.54 | 8.52 | n.t. | 5.71 | n.t. | ||||||||||
t5 min | 5.53 | 8.40 | 7.03 | 5.82 | |||||||||||
GDA (ppm) | 125 | 4.88 | 0.65±0.34 | 7.96 | 0.44±0.38 | 6.57 | 0.46±0.36 | 5.38 | 0.43±0.23 | 0.48±0.65 | 0.49±0.41 | 0.52±0.55 | |||
500 | 2.93 | 2.61±0.67 | 6.88 | 1.52±0.38 | 5.87 | 1.17±0.39 | 3.91 | 1.92±0.26 | 1.31±0.57 | 2.09±0.35 | 2.52±0.58 | ||||
1000 | 2.19 | 3.34±1.02 | 5.90 | 2.50±0.38 | 5.22 | 1.81±0.29 | 3.00 | 2.82±0.75 | 1.73±1.11 | 2.71±0.39 | 2.98±0.58 | ||||
2000 | 1.52 | 4.01±0.27 | 5.15 | 3.25±0.35 | 3.91 | 3.13±0.31 | 1.98 | 3.84±0.43 | 3.32±0.64 | 2.88±0.36 | 3.05±0.53 | ||||
2500 | 1.50 | 4.03±0.26 | 4.09 | 4.31±0.36 | 3.10 | 3.94±0.38 | 1.95 | 3.87±0.58 | 3.65±0.79 | 3.61±0.35 | 3.05±0.53 | ||||
control | inocculum | 6.25 | 8.44 | n.t. | 6.7 | n.t. | 7.06 | 5.66 | |||||||
t0 min | 5.81 | 8.57 | n.t. | 6.17 | n.t. | 7.32 | 5.61 | ||||||||
t5 min | 6.00 | 8.56 | 6.86 | 6.11 | 7.11 | 7.55 | 5.55 | ||||||||
PAA (ppm) | 50 | 5.77 | 0.23±0.41 | 8.25 | 0.31±0.35 | 6.23 | 0.63±0.63 | 5.12 | 0.99±0.35 | 6.46 | 0.65±1.06 | 6.84 | 0.71±0.37 | 5.50 | 0.04±0.59 |
200 | 4.90 | 1.10±0.32 | 7.79 | 0.77±0.35 | 5.48 | 1.38±0.50 | 2.41 | 3.70±0.78 | 3.21 | 3.90±1.00 | 5.80 | 1.75±0.40 | 5.11 | 0.44±0.59 | |
500 | 3.92 | 2.08±0.37 | 6.90 | 1.67±0.35 | 4.73 | 2.13±0.37 | 2.10 | 4.01±1.04 | 1.67 | 5.44±1.02 | 5.56 | 1.99±0.37 | 4.78 | 0.77±0.65 | |
1000 | 3.07 | 2.94±0.27 | 6.32 | 2.25±0.35 | 3.11 | 3.75±0.57 | 1.79 | 4.32±0.69 | 1.61 | 5.50±0.98 | 4.98 | 2.57±0.34 | 3.90 | 1.65±0.59 | |
1500 | 2.69 | 3.31±0.39 | 6.00 | 2.56±0.34 | 2.59 | 4.27±0.34 | 1.76 | 4.35±0.55 | 1.50 | 5.61±0.97 | 4.84 | 2.71±0.39 | 3.57 | 1.98±0.60 | |
control | inocculum | 5.80 | 8.25 | n.t. | 5.42 | n.t. | 7.40 | 5.75 | |||||||
t0 min | 5.50 | 8.42 | n.t. | 5.57 | n.t. | 7.69 | 5.71 | ||||||||
t5 min | 5.88 | 8.38 | 6.95 | 5.72 | 5.90 | 7.65 | 5.63 | ||||||||
ethanol (v/v) | 10% | 5.36 | 0.52±0.97 | 8.52 | −0.14±0.38 | 6.51 | 0.44±0.24 | 5.88 | −0.16±0.40 | 5.85 | 0.05±0.28 | 7.04 | 0.61±0.39 | 5.36 | 0.27±0.51 |
20% | 5.64 | 0.24±0.98 | 8.42 | −0.04±0.38 | 6.39 | 0.56±0.33 | 5.69 | 0.03±0.29 | 5.77 | 0.13±0.27 | 6.84 | 0.81±0.39 | n.t. | n.t. | |
30% | 5.60 | 0.28±0.72 | 8.32 | 0.06±0.37 | 6.03 | 0.91±0.37 | 5.50 | 0.22±0.37 | 5.77 | 0.13±0.34 | 6.82 | 0.83±0.41 | 5.65 | −0.02±0.49 | |
40% | 5.41 | 0.47±0.86 | 8.29 | 0.08±0.37 | 6.20 | 0.75±0.41 | 5.50 | 0.22±0.37 | 5.62 | 0.28±0.31 | 6.71 | 0.94±0.38 | n.t. | n.t. | |
50% | 5.36 | 0.52±0.64 | 8.27 | 0.10±0.36 | 6.05 | 0.90±0.26 | 4.69 | 1.03±0.50 | 5.32 | 0.58±0.27 | 6.69 | 0.96±0.40 | 5.21 | 0.42±0.47 | |
control | 5.80 | 8.25 | n.t. | 5.42 | n.t. | 7.40 | 5.80 | ||||||||
5.79 | 8.42 | n.t. | 5.64 | n.t. | 7.69 | 5.79 | |||||||||
5.79 | 8.38 | 6.76 | 6.07 | 5.90 | 7.65 | 5.79 | |||||||||
1-pro-panol (v/v) | 10% | 5.88 | −0.09±1.31 | 8.56 | −0.18±0.32 | n.t. | n.t. | n.t. | 5.63 | 0.27±0.17 | 7.19 | 0.46±0.39 | 5.88 | −0.09±1.31 | |
20% | 6.12 | −0.33±1.34 | 8.42 | −0.04±0.34 | 6.66 | 0.10±0.25 | 5.60 | 0.47±0.66 | 5.72 | 0.18±0.41 | 7.05 | 0.60±0.40 | 6.12 | −0.33±1.34 | |
30% | 5.79 | 0.00±0.65 | 8.34 | 0.04±0.36 | 6.47 | 0.29±0.20 | 5.98 | 0.09±0.82 | 5.77 | 0.13±0.36 | 6.98 | 0.67±0.40 | 5.79 | 0.00±0.65 | |
40% | 5.79 | 0.00±0.65 | 8.29 | 0.09±0.36 | 6.41 | 0.35±0.16 | 5.41 | 0.66±0.52 | 5.60 | 0.30±0.41 | 6.92 | 0.73±0.38 | 5.79 | 0.00±0.65 | |
50% | 5.45 | 0.33±0.67 | 8.25 | 0.13±0.35 | 6.41 | 0.35±0.22 | 5.69 | 0.38±0.66 | 5.88 | 0.02±0.57 | 6.82 | 0.83±0.41 | 5.45 | 0.33±0.67 | |
60% | 5.55 | 0.24±1.28 | 8.13 | 0.25±0.32 | 6.45 | 0.31±0.24 | 5.31 | 0.76±0.66 | 5.21 | 0.35±0.55 | 6.75 | 0.90±0.37 | 5.55 | 0.24±1.28 | |
control | 5.80 | 8.25 | n.t. | 5.42 | n.t. | 7.40 | 5.75 | ||||||||
5.50 | 8.42 | n.t. | 6.15 | n.t. | 7.69 | 5.71 | |||||||||
5.88 | 8.38 | 6.99 | 5.86 | 5.60 | 7.65 | 5.63 | |||||||||
2-pro-panol (v/v) | 20% | 5.64 | 0.24±0.96 | 8.67 | −0.29±0.35 | 6.43 | 0.56±0.41 | 5.98 | −0.12±0.68 | 5.52 | 0.08±0.12 | 7.29 | 0.36±0.41 | n.t. | n.t. |
30% | 6.02 | −0.14±1.17 | 8.52 | −0.14±0.35 | 6.49 | 0.50±0.30 | 5.93 | −0.07±0.83 | 5.38 | 0.22±0.31 | 7.23 | 0.42±0.42 | n.t. | n.t. | |
40% | 5.98 | −0.10±1.19 | 8.40 | −0.02±0.36 | 6.32 | 0.67±0.27 | 5.83 | 0.03±0.74 | 5.40 | 0.20±0.26 | 7.17 | 0.48±0.43 | 5.46 | 0.17±0.56 | |
50% | 5.52 | 0.36±0.72 | 8.29 | 0.09±0.34 | 6.22 | 0.77±0.29 | 5.45 | 0.41±0.74 | 5.33 | 0.27±0.22 | 7.02 | 0.63±0.41 | 5.65 | −0.02±0.52 | |
60% | 5.38 | 0.50±0.86 | 8.03 | 0.35±0.33 | 6.30 | 0.69±0.24 | 5.50 | 0.36±0.66 | 5.47 | 0.13±0.18 | 6.92 | 0.73±0.40 | 5.25 | 0.38±0.53 |