Table 8. Mutation patterns in LPV/r failing C patients from Israel and South Africa.

Mut/Seq	Mutation Patterns	Number found	Origin (No.)
1	M46I	2	SA(1), IL(1)	Most common patterns
1	I47A/V	2	SA(1), IL(1)
1	V82A	2	SA(1), IL(1)
1	L90M	7	SA(1), IL(7)	Patterns	Actual frequency	Calculated frequency	p
2	L10F, V82A	5	SA(4), IL(1)
2	M46I, L76V	2	SA(2)
2	I54V, V82A	7	SA(6), IL(1)	M46I+L76V	0.19	0.12	0.2
3	L10F, I54V, V82A	2	IL(2)	M46I+V82A	0.47	0.37	0.2
3	M46I, I54V, V82A	6	SA(3), IL(3)	I54V+V82A	0.6	0.47	0.09
3	I54V, L76V, V82A	2	SA(2)	M46I+I54V+V82A	0.44	0.25	0.01
4	L10F, L33F, I54V, V82A	2	SA(1), IL(1)	M46I+I54V+L76V	0.17	0.08	0.09
4	L10F, M46I, I54V, V82A	4	SA(2), IL(2)	M46I+L76V+V82A	0.16	0.09	0.2
4	M46I, I50V, I54V, V82A	2	SA(2)	I54V+L76V+V82A	0.19	0.11	0.2
4	M46I, I54V, L76V, V82A	4	SA(3), IL(1)	M46I+I54V+L76V+V82A	0.16	0.06	0.04
5	L10F, M46I, I54V, L76V, V82A	7	SA(4), IL(3)
6	L10F, L24I, L33F, M46I, I54V, V82A	3	SA(2), IL(1)
Total	16	59

The table lists all mutation patterns that appeared more than once in the combined dataset of 101 samples, 55 from South Africa (van Zyl et al. [33]) and 46 from Israel (this study). Fifty nine samples (58% of total) included 16 patterns. The actual frequency (number of times a pattern appeared in the dataset) and the calculated frequency (based on the overall frequencies of each mutation included in that pattern, assuming independence) for the most prevalent patterns are compared. p-values were calculated using Fisher's two- tailed Exact Test. IL – Israel; SA – South Africa.