Table 1. Host range of Tsamsa phage on 43 different Bacillus strains and 12 non-Bacillus strains and lytic spectrum of Tsamsa endolysin for a subset of the Bacillus strains. Presence of lysis is indicated by+and absence of lysis is indicated by -. n.d.: not determined.
Strain name | Organism | Notes | Source | Phage lysis | Endolysin lysis |
6602 R1 | Bacillus anthracis | pXO1-pXO2 negative | [39] | + | + |
Sterne | Bacillus anthracis | pXO2 negative | Institut Pasteur #7702 | + | + |
Weybridge UM44 | Bacillus anthracis | pXO2 negative | [39] | + | + |
Ames-non reverting | Bacillus anthracis | pXO2 negative | U.S. Dept. of Agriculture, Ames, Iowa | + | + |
Ames | Bacillus anthracis | U.S. Dept. of Agriculture, Ames, Iowa | + | n.d | |
Vollum 1b | Bacillus anthracis | Laboratory Strain | + | n.d. | |
PAK-1 | Bacillus anthracis | Pakistan isolate, M. Hugh-Jones collection | − | n.d. | |
569 | Bacillus cereus | [17] | + | n.d. | |
LA 925 | Bacillus cereus | CHUV | − | + | |
ATCC 14579 | Bacillus cereus | ATCC | − | + | |
ATCC 11778 | Bacillus cereus | ATCC | + | + | |
ATCC 10702 | Bacillus cereus | ATCC | − | + | |
ATCC 10876 | Bacillus cereus | ATCC | + | n.d. | |
DSM 2302 | Bacillus cereus | DSM | − | + | |
BO 366 | Bacillus cereus | This study | − | − | |
BO 372 | Bacillus cereus | This study | − | − | |
BO 493 | Bacillus cereus | This study | − | − | |
DSM 4218 | Bacillus cereus | DSM | − | + | |
ATCC 33019 | Bacillus cereus | ATCC | + | + | |
ATCC 14737 | Bacillus cereus | ATCC | + | + | |
DSM1274 | Bacillus cereus | DSM | − | + | |
ATCC 27522 | Bacillus cereus | ATCC | − | + | |
NCTC 11143 | Bacillus cereus | NCTC | − | + | |
NCIMB 8705 | Bacillus cereus | NCIMB | + | + | |
ATCC 6464 | Bacillus cereus | ATCC | − | + | |
B346 | Bacillus cereus | Mouse isolate | − | + | |
DSM360 | Bacillus cereus | DSM | − | − | |
HER1399 | Bacillus cereus | HER | + | + | |
WSBC 10530 | Bacillus cereus | WSBC | − | − | |
WSBC 10556 | Bacillus cereus | WSBC | − | + | |
WSBC 10566 | Bacillus cereus | WSBC | − | − | |
WSBC 10583 | Bacillus cereus | WSBC | − | − | |
DSM4421 | Bacillus thuringiensis | DSM | − | + | |
WSBC 10204 | Bacillus thuringiensis | WSBC | − | − | |
HER1211 | Bacillus thuringiensis | HER | + | + | |
Kurstaki | Bacillus thuringiensis | Industry isolate | − | − | |
ATCC 10792 | Bacillus thuringiensis | ATCC | + | + | |
DSM168 | Bacillus subtilis | DSM | − | − | |
DSM675 | Bacillus subtilis | DSM | − | − | |
ATCC 23059 | Bacillus subtilis | ATCC | − | n.d. | |
DSM395 | Bacillus sphaericus | DSM | − | − | |
DSM90 | Bacillus megaterium | DSM | − | − | |
WSBC 10550 | Bacillus weihenstephanensis | WSBC | − | + | |
WSLC 3009 | Listeria ivanovii | WSLC | − | ||
ATCC BAA-679 | Listeria monocytogenes | ATCC | − | ||
PSK | Staphylococcus aureus | Laboratory Stock | − | ||
Twort | Staphylococcus aureus | Laboratory Stock | − | ||
414 | Staphylococcus epidermidis | Laboratory Stock | − | ||
100655 | Staphylococcus epidermidis | Laboratory Stock | − | ||
602 | Staphylococcus epidermidis | Laboratory Stock | − | ||
DT7155 | Salmonella Typhimurium | Laboratory Stock | − | ||
CGSC 4401 | Escherichia coli | CGSC | − | ||
DSM 20560 | Streptococcus salivarius | DSM | − | ||
NZ9000 | Lactococcus lactis | Laboratory Stock | − | ||
ATCC 19433 | Enterococcus faecalis | ATCC | − |
Source abbreviations: CHUV: Strain collection of the Centre Hospitalier universitaire Vaudois, Switzerland; HER: Félix d’Hérelle Reference Center for bacterial viruses, Laval, Canada; ATCC: American Type Culture Collection; NCTC: National Collection of Type Cultures; DSM: Deutsche Sammlung von Mikroorganismen; NCIMB: National Collection of Industrial Bacteria; WSBC: Weihenstephan Bacillus Collection; WSLC = Weihenstephan Listeria Collection; CGSC: Coli Genetic Stock Center, Yale, USA.