. 2014 Jan 27;9(1):e86230. doi: 10.1371/journal.pone.0086230

Table 1. The kinetic and inhibition profile of 8-methoxypsoralen on coumarin 7-hydroxylase activity of the wild type CYP2A6, CYP2A615, CYP2A616, CYP2A621 and CYP2A622.

CYP Protein	K_m (µM)	IC₅₀ (µM)	Fold
*CYP2A61 (wild-type)**	10.61±1.58	0.19±0.05	1
*CYP2A615**	22.13±1.29**	2.39±0.0**	12.58
*CYP2A616**	10.53±1.45	0.43±0.1	2.26
*CYP2A621**	16.39±0.41**	1.56±0.21**	8.21
*CYP2A622**	31.16±2.22**	1.05±0.21**	5.53

All values are shown as the means ± SD of three independent determinations. The K_m values were determined in our previous study [9] and listed here for comparison purpose. The IC₅₀ values were determined in the present studies. The last column represents the fold of change in IC₅₀ relative to the wild type. Degree of statistical differences between the wild-type and the mutants as determined by one-way analysis of variance: the mean difference is significant at 0.05 level (* p<0.05) and highly significant at 0.01 level (** p<0.01).

Table 1. The kinetic and inhibition profile of 8-methoxypsoralen on coumarin 7-hydroxylase activity of the wild type CYP2A6, CYP2A6*15, CYP2A6*16, CYP2A6*21 and CYP2A6*22.

Table 1. The kinetic and inhibition profile of 8-methoxypsoralen on coumarin 7-hydroxylase activity of the wild type CYP2A6, CYP2A615, CYP2A616, CYP2A621 and CYP2A622.