Figure 2. Drought-responsive genes in soybean exhibit diurnal regulation, and the expression pattern is modified under drought stress.

Gene expression data regards qPCR analysis of soybean leaves during moderate (A) and severe (B) drought. Expression axis represents normalized expression (NE) = 2∧^{-(Ct experimental – Ctn)}. Collect time points are represented by ZT (Zeitgeiber Time) 0 to 20, starting from the time the lights came on ( ZT0) and proceeding with 4 h intervals until ZT20. For easy viewing, asterisks represent significant differences between control and stressed plants in each time point (Duncan’ test 5%, time -treatment interaction). The ANOVA and the complete Duncan’s tests results can be found in Table S3.