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. 2014 Jan 27;9(1):e86421. doi: 10.1371/journal.pone.0086421

Figure 2. Cell death and caspase activation caused by nsPEFs are dose dependent in vitro.

Figure 2

Cells were harvested with trypsin and re-suspended in fresh medium with 10% FBS and then placed in 0.2 cm gap cuvettes. They were exposed to ten 100 nsPEFs from 0 to 60 kV/cm. 24 h survival cell were counted and normalized to control cells as 100% (Figure 2A). The different cell lines of HCC showed different nsPEFs-vulnerability (hep1-6>HepG2>SMMC7721>HCCLM3), but all of their cell death rate increased when the nsPEFs raised from 0, 10, 20, 30, 40, 50 to 60 kV/cm. The caspase activation rate was also dependent on the nsPEFs strength (Figure 2C the caspase activation rate of HepG2). Similar results were also observed in SMMC7721, HCCLM3 and Hep1-6 cells (data not shown).